1


Contents
dictyostelium Developement
Freezing Cells
Freezing Spores
Bringing up stocks from freezer
Setting up on Filter pads for development
Media
HL-5 media
Agar plates - SM 1L
LPS Buffer
Development drugs
Geneticin (G418) (Sigma #G9516)
Blasticidin (Sigma #15205)
Microscopy
Multi-photon confocal
Zeiss Axiovert 3D imaging scope
Experiments
Natural wave
pulsing experiment
Mixing Experiment
Fluorescein for concentration testing
Fixatives
paraformeldahyde
fix for 3D imaging GFP
Cytoskeleton Buffer CB (MES, Krammer buf)
Untitled
Computer Administration
Keck2 server
Keck Readin Computers
Keck Terminal Computers (workstations)
Backup system
Pump Programing
Untitled
dictyostelium Strains
Ax3
Ax2
pten-
ax3-abd-gfp
myoII GFP
Apparatus
Timer setup (white newer timer, green air products)